[2021-04-15 15:37:58] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2021-04-15 15:37:58] Checking for Bowtie Bowtie version: 2.4.2.0 [2021-04-15 15:37:58] Checking for Bowtie index files (genome).. [2021-04-15 15:37:58] Checking for reference FASTA file [2021-04-15 15:37:58] Generating SAM header for ref/test_ref [2021-04-15 15:37:58] Preparing reads left reads: min. length=75, max. length=75, 100 kept reads (0 discarded) [2021-04-15 15:37:58] Mapping left_kept_reads to genome test_ref with Bowtie2 [2021-04-15 15:37:58] Mapping left_kept_reads_seg1 to genome test_ref with Bowtie2 (1/3) [2021-04-15 15:37:58] Mapping left_kept_reads_seg2 to genome test_ref with Bowtie2 (2/3) [2021-04-15 15:37:58] Mapping left_kept_reads_seg3 to genome test_ref with Bowtie2 (3/3) [2021-04-15 15:37:58] Searching for junctions via segment mapping [2021-04-15 15:37:58] Retrieving sequences for splices [2021-04-15 15:37:58] Indexing splices [2021-04-15 15:37:59] Mapping left_kept_reads_seg1 to genome segment_juncs with Bowtie2 (1/3) [2021-04-15 15:37:59] Mapping left_kept_reads_seg2 to genome segment_juncs with Bowtie2 (2/3) [2021-04-15 15:37:59] Mapping left_kept_reads_seg3 to genome segment_juncs with Bowtie2 (3/3) [2021-04-15 15:37:59] Joining segment hits [2021-04-15 15:37:59] Reporting output tracks ----------------------------------------------- [2021-04-15 15:37:59] A summary of the alignment counts can be found in out/tophat_se/align_summary.txt [2021-04-15 15:37:59] Run complete: 00:00:01 elapsed