[M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::process] read 800 sequences (80000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 298, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 178, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 234) [M::mem_pestat] mean and std.dev: (178.00, 17.68) [M::mem_pestat] low and high boundaries for proper pairs: (102, 256) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 800 reads in 0.868 CPU sec, 0.234 real sec [main] Version: 0.7.17-r1188 [main] CMD: bwa mem -a -M -t 4 /project/scv/examples/bioinformatics/gatk/ref/Homo_sapiens_assembly38.fasta /project/scv/examples/bioinformatics/gatk/data/SRR062634_X400/SRR062634_1.fastq /project/scv/examples/bioinformatics/gatk/data/SRR062634_X400/SRR062634_2.fastq [main] Real time: 47.255 sec; CPU: 4.844 sec