[M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::process] read 400 sequences (40000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 147, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 177, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (132, 227) [M::mem_pestat] mean and std.dev: (180.61, 14.23) [M::mem_pestat] low and high boundaries for proper pairs: (113, 246) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 400 reads in 1.161 CPU sec, 0.582 real sec [main] Version: 0.7.17-r1188 [main] CMD: bwa mem -a -M -t 2 /project/scv/examples/bioinformatics/gatk/ref/Homo_sapiens_assembly38.fasta /project/scv/examples/bioinformatics/gatk/data/SRR062634_X200/SRR062634_1.fastq /project/scv/examples/bioinformatics/gatk/data/SRR062634_X200/SRR062634_2.fastq [main] Real time: 48.615 sec; CPU: 6.254 sec